Structured illumination temporal compressive microscopy.
نویسندگان
چکیده
We present a compressive video microscope based on structured illumination with incoherent light source. The source-side illumination coding scheme allows the emission photons being collected by the full aperture of the microscope objective, and thus is suitable for the fluorescence readout mode. A 2-step iterative reconstruction algorithm, termed BWISE, has been developed to address the mismatch between the illumination pattern size and the detector pixel size. Image sequences with a temporal compression ratio of 4:1 were demonstrated.
منابع مشابه
Nonlinear structured-illumination enhanced temporal focusing multiphoton excitation microscopy with a digital micromirror device
In this study, the light diffraction of temporal focusing multiphoton excitation microscopy (TFMPEM) and the excitation patterning of nonlinear structured-illumination microscopy (NSIM) can be simultaneously and accurately implemented via a single high-resolution digital micromirror device. The lateral and axial spatial resolutions of the TFMPEM are remarkably improved through the second-order ...
متن کاملFluorescent microscopy beyond diffraction limits using speckle illumination and joint support recovery
Structured illumination microscopy (SIM) breaks the optical diffraction limit by illuminating a sample with a series of line-patterned light. Recently, in order to alleviate the requirement of precise knowledge of illumination patterns, structured illumination microscopy techniques using speckle patterns have been proposed. However, these methods require stringent assumptions of the speckle sta...
متن کاملStructured Illumination by a Pinhole Array for Holographic Microscopy
The structured illumination of samples is known as one of the techniques for sub diffraction optical microscopy [1-3]. A spatial resolution beyond the optical diffraction limit is achievable by using nano light spots for scanning of the sample. We apply the structured illumination for digital inline holographic microscopy [4-6]. The structured illumination patterns are superposed by that of the...
متن کاملPlasmonic structured illumination microscopy.
We propose a super resolution imaging technique called plasmonic structured illumination microscopy (PSIM), which combines the structured illumination microscopy technique with the tunable surface plasmon interference. Because of the high-resolution enabled by using surface plasmon interference as an illumination source, PSIM possesses higher image resolving power compared with conventional str...
متن کاملLaser Illumination in Live Cell Microscopy: Scattering and Structured Illumination
Two types of laser illumination in live cell microscopy with a focus on the sample or in the aperture plane of the microscope objective lens are distinguished. For the second case two examples are described, namely light scattering microscopy with angular resolution and Structured Illumination Microscopy (SIM) with two interfering laser beams. Appropriate applications include morphological stud...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Biomedical optics express
دوره 7 3 شماره
صفحات -
تاریخ انتشار 2016